News + Events
- Aby Joseph, Andres Guevara, and Jesse Schallek describe a new, noninvasive approach to assess vascular health in the journal eLife. Schallek's lab, part of the Flaum Eye Institute, developed a method to visualize how single blood cells flow through vessels of the eye using adaptive optics imaging.
+ Congratulations to Dr. Sarah Walters, for her successful PhD Defense, "Two-Photon Excited Fluorescence Adaptive Optics Ophthalmoscopy of
Retinal Function." Advised by Prof. Jennifer Hunter & Prof. David Williams
Abstract: The retina is the light-sensitive tissue at the back of the eye, which carries out the first steps in vision. Specialized neural cells in the retina known as photoreceptors are responsible for detection of light and its transduction by initiating an electrical signal to the brain. Adaptive optics scanning light ophthalmoscopy (AOSLO), which dynamically corrects aberrations of the ocular media in the living eye and affords a lateral resolution of 2 μm, has revolutionized our ability to visualize photoreceptors and many other microstructures in the retina. The implementation of two-photon excited fluorescence (TPEF) imaging in AOSLO has enabled not only complementary structural information throughout the retina, but an objective, non-invasive measure of visual function in photoreceptors by measuring TPEF kinetics from these cells.
The aim of the present thesis is to further develop and apply TPEF ophthalmoscopy as a novel measure of in vivo cellular function in the retina. First, TPEF ophthalmoscopy was used in conjunction with other imaging modalities to evaluate the extent of photoreceptor dysfunction in a non-human primate model of retinal degeneration. TPEF ophthalmoscopy was essential in determining that photoreceptors were non-functional. Second, the sensitivity of TPEF kinetics to detect changes in photoreceptor function in conditions relevant to disease pathogenesis was investigated. Systemic hypoxia was employed in non-human primates as a model of physiological change, reducing oxygen supply to the retina, and TPEF kinetics were shown to be slowed as a consequence. Finally, the capabilities of TPEF ophthalmoscopy were expanded by implementing intrinsic fluorescence lifetime imaging. TPEF lifetime imaging was shown to distinguish retinal cell classes that are functionally disparate, and lifetimes were altered in regions of retinal damage.
TPEF ophthalmoscopy has the potential to yield advances in understanding of both the basic physiology and pathology of the retina. If translated successfully into humans, TPEF ophthalmoscopy demonstrates promise as a valuable imaging modality that may, when used in conjunction with other clinical measures, identify early cellular dysfunction and longitudinally track pathological changes. Ultimately, it may assist in timely diagnosis, intervention, and development of treatments or vision restoration methods to combat blindness as a consequence of retinal disease.
- Congratulations to Tyler Godat and Khang Huynh, who received the ARVO Foundation/Retina Reseach Foundation/Joseph M. and Eula C. Lawrence Travel Grant to the 2019 ARVO Annual Meeting in Vancouver, British Columbia.
- Aby Joseph and Sarah Walters received the ARVO Foundation/Retina Reseach Foundation/Joseph M. and Eula C. Lawrence Travel Grant to the 2018 ARVO Annual Meeting in Honolulu, HI
- Congratulations to Juliette McGregor, winner of the 2018 OSA FVM Young Investigator Award.
- July's Hajim Spotlight: Jennifer Hunter
- ‘Bionic Eye’ Helps Wyoming County Man See His Bride Again
- Congratulations to Aby Joseph, who won the best graduate student poster prize at the UR annual neuroscience retreat!
- Congratulations to Aby Joseph, winner of the 2017 Leica Imaging Award for his time-lapse imaging of retinal microglia. Aby was honored with the award at the 2017 Immune Imaging Symposium at the University of Rochester.
- A 71-year-old woman blinded by an inherited disease recently thrilled University of Rochester doctors by reaching out and grabbing her ophthalmologist’s hand after receiving a “bionic eye.” The device allows her to distinguish light and motion, which she hasn’t been able to do in decades. Read more...
- Congratulations to Ge Song, winner of the President's award for Engineering and Applied Sciences at the Undergraduate Research Exposition.
- In a study highlighted in the Proceedings of the National Academy of Sciences, Ethan A. Rossi, Ph.D., assistant professor of Ophthalmology at the University of Pittsburgh School of Medicine, describes a new method to non-invasively image the human retina, a layer of cells at the back of the eye that are essential for vision. The group, led by David Williams, Ph.D., Dean for Research in Arts, Sciences, and Engineering and the William G. Allyn Chair for Medical Optics at the University of Rochester, was able to distinguish individual retinal ganglion cells (RGCs), which bear most of the responsibility of relaying visual information to the brain. Read more...
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